; s.c. 0.7 (26.9) I.U.Syrian hamsters: i.v. Equines Choriongonadotropin wird in den maternalen Blutkreislauf sezerniert, um auf die Ovarien der Stute einen LH-ähnlichen Effekt auszuüben. Deshalb wird angenommen, dass nur die LH-ähnliche Komponente des equinen Choriongonadotropins bei der trächtigen Stute eine funktionelle Bedeutung hat. Our global footprint enables us to serve more than 225,000 customer locations and gives us extensive access to research laboratories and scientists in more than 180 countries. eCG. In many cases a COA can be faxed VWR is your complete source for workplace supplies. Quanta Biosciences and VWR are proud to fund a grant award for publication of research involving qPCR methodologies utilizing any Quanta Bioscience qPCR reagent in JoVE. Place each female into a cage with one stud male after the administration of hCG. Equines Choriongonadotropin kann sowohl subkutan, als auch intramuskulär oder intravenös appliziert werden. It is important to watch carefully and move the eggs from the hyaluronidase solution as soon as the cumulus cells get loose to avoid damaging the eggs. Your session has expired. ), or subcutaneous (s.c.) methodstet, the effective dose values for 50% of the animals (ED50) per animal and per body weight (kg) in parenthesis were as follows: Mice: i.v. All these parameters should be considered together to achieve an efficient response to superovulation with all conditions optimized empirically in each actual setting. One of our greatest strengths comes from having a global infrastructure that is strategically located to support the needs of our customers. The use of eCG determinations as a positive pregnancy diagnosis test will consequently yield some mares to have been diagnosed pregnant but not delivering a foal (Fig. Nathalie Eisenhardt, ... Wolfram Antonin, in Methods in Cell Biology, 2014, 240-IU/ml pregnant mare serum gonadotropin (PMSG, available as Intergonan, Intervet), 1000-IU/ml human chorionic gonadotropin (HCG, available as Ovogest, Intervet), Beckman L-60 Ultracentrifuge, SW55 Ti swinging bucket rotor and ultraclear tubes (or equivalents), Beckman Optima TLX Ultracentrifuge, TLA120.2 rotor and tubes (or equivalent system), Häreus-Multifuge 1-LR-Centrifuge (or equivalent), 5 ml syringes, 27G 3/4 in. In some species and circumstances, it is more important, however, to induce ovulation, rather than superovulation; this is true of animals that show seasonal periods of anovulation or in women who are anovulatory because of events of known or unknown etiology. Figure 7.16. Questions? The zonae pellucidae around the swollen areas of collected MII oocytes are cut as shown above in M2 medium without cytoskeleton inhibitors at 37°C (Figure 7.13B–E). Copulation is confirmed by presence of a vaginal plug in the female next morning. Used under license. This hormone is composed of two non-covalently linked α and β subunits. 1988). per female mouse (3–8 weeks of age) using a 26-gauge needle (e.g., B-D, 305111) attached to a 1 or 3 cc syringe. 100355-394EA 265.35 USD. Equine Gonadotropin, Pregnant Mares′ Serum (PMS-G) is used in conjunction with Human chorionic gonadotropin (hCG, Product No. (A) Metaphase chromosomes are removed by an enucleation pipette from the slit of zona (arrowhead shows chromosomes). Product is qualified for use in mouse embryo preparation. Need larger quantities for your development, manufacturing or research applications? search was unable to find the COA you are looking for, you will be Easy access to products and protocols for research use only in the identification of 2019-nCoV based on Centers for Disease Control and Prevention (CDC) recommendations. EC Number 232-663-9. C 8554) to superovulate female mice. Gynäkologie, Responsiveness of BALB/c and congenic strains developed with BALB/c mice is generally poorer with most uniform results obtained with 12-week-old donors as opposed to a greater uniformity and yield from 3- to 5-week-old donors using most other strains. 2.0 (76.9) I.U. If you find a lot number with a filling-code such as �-#q��C���Y��u��|wh�v$ �tV�uZ|� ���p��x�ds��4�y�[����$a A����[��T��t����Ks�����=����-l�� ������X�"j��ރ��D���hd�&}��+�؇���kJCf�T�!���8:iI����R�LAL�)^�J6�^�N9P�� 5��C�z��fQO'5�r"I�5���� �����aڢl��;��� 3.5 (12.3) I.U. Es besteht die Theorie, dass das apparente refraktäre Verhalten von Stuten gegenüber ihrem eigenen Hormon deshalb entwickelt wurde, um die Ovarien während der Trächtigkeit vor einer Überstimulation zu schützen. C 8554) to superovulate female mice. 0.8 (30.8) I.U. The electric pulses protocol has proved effective in our experience. The timing of the injections will influence the success as well, and most importantly, it will determine the stage at which the resulting embryos will have reached at the time of recovery. Equines Choriongonadotropin kann aus dem Serum trächtiger Stuten gewonnen werden. Es besteht zu einem hohen Anteil aus Kohlenhydraten (ca. Wash the cumulus cell-free eggs through several drops of Whitten's/HEPES/PVA and then transfer them to a culture dish containing microdrops of Whitten's/PVA covered with light paraffin oil. After the electric pulses, reconstituted oocytes are cultured in M16 medium for 5 h and examined for pronuclear-like formation of donor nucleus derivation in ooplasm.

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